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SCIENTIFIC INTEREST

Michael is an internationally recognised expert in the field of transcriptomics, next-generation sequencing technologies and bioinformatics. His current scientific interest focuses on investigations of gene expression and alternative splicing patterns in distinct structures and cell types of the healthy human brain and perturbation of transcriptome profiles during the onset and progression of neurological disorders including multiple system atrophy, epilepsy, amyotrophic lateral sclerosis and chronic fatigue syndrome. Michael is also interested in exploration of cancer-specific alterations in transcriptome profiles towards identification of novel biomarkers such as circular and non-protein coding RNAs.

RESEARCH CONTRIBUTIONS

During his PhD studies and subsequently as a postdoc at the German Centre for Rheumatism Research, Michael specialised in investigating the influence of the sequence polymorphism within the promoter regions of MHC class II genes in several inbred mice strains (Janitz et al. 1997; Janitz et al. 1998; Cowell et al. 1998).

Joining the Max Planck Institute for Molecular Genetics (MPIMG) converged with Michael’s growing interest in studying transcription at the genome-wide level. Amongst others, he was involved in collaborative projects to characterise cDNA sequences on the level of the whole transcriptome in mice T helper cells and bovine brain (Gutjahr et al. 2005; Jann et al. 2006), respectively.

While at the MPIMG, Michael and his research group focused on developing a transfected-cell array as a high-throughput genomic tool for functional analysis of genes and their products (Vanhecke and Janitz 2004). This resulted in application of the cell arrays for subcellular protein localisation studies (Hu et al. 2006; Hu et al. 2009; Hu et al. 2010), protein-protein interaction screens (Fiebitz et al. 2008), and functional promoter analysis (Cheng et al. 2010). In addition, with collaborative partners in national and European Community research programs, he applied gene expression profiling studies to identify the genes involved in T helper lymphocytes type 1 immune response (Niesner et al. 2008) and differentiation of murine palatal development (Nogai et al. 2008). His research group also developed miniaturised microarray platforms for DNA hybridization studies using PNA- (Bauer et al. 2004) and LNA-modified oligonucleotide probes (Guerasimova et al. 2006; Liu et al. 2006 and 2007), thus contributing to more efficient exploration of the genome structure and function.

CURRENT RESEARCH

Since his appointment at լ�и��� Michael has been focused on exploration of different segments of human and mouse transcriptome using combination of Illumina and nanopore DNA and RNA sequencing as well as unique analytical pipeline combining in-house and publicly available bioinformatics tools. He and his team provided first insights into non-protein coding transcriptome of different regions of the human cortex, both in health (Mills et al. 2013; Mills et al. 2015a; Mills et al. 2015b; Bliim et al. 2019) and neurological diseases such as Alzheimer’s disease (Twine et al. 2011; Mills et al. 2013; Mills et al. 2014), multiple system atrophy (MSA)(Mills et al. 2015c; Mills et al. 2016) and epilepsy (Mills et al. 2020) as well as cancer (Takenaka et al. 2016; Chen et al. 2017a). His recent research has been concentrated on circular RNAs (circRNAs) genome-wide expression patterns in the normal human (Chen et al. 2019) and mouse brain (Chen et al. 2018) as well as MSA (Chen et al. 2016) and endometrial cancer (Chen et al. 2017b).

Current research projects aim at understanding of circRNA-miRNA-mRNA networks in epilepsy and amyotrophic lateral sclerosis as well as gynaecological malignancies. Another emerging avenue of Michael’s research is exploration of RNA post-transcriptional modifications in healthy human tissues and cancer using nanopore direct RNA sequencing. Michael’s quest for the discovery of circRNAs, which might serve as molecular biomarker for MSA and endometrial cancer, constitutes another important element of his research program.

COLLABORATIVE PROJECTS

• Investigation of circular expression patterns in mesial temporal lobe epilepsy; in collaboration with Dr James Mills and Prof. Eleonora Aronica form University of Amsterdam, The Netherland and Prof Orrin Devinsky from New York University Langone Medical Center, U.S.A. 
• Determination of the transcriptome perturbation in endometrial cancer; in collaboration with A/Prof Kyle Hoehn from School of BABS, լ�и��� Australia and A/Prof Rhonda Farrell from Prince of Wales Private Hospital Sydney.
• Investigation of changes in circular transcriptome patterns in amyotrophic lateral sclerosis; in collaboration with Dr Sam Brennan and Dr Matt Keon from GenieUs Genomics Pty Ltd, Sydney, Australia.
• Investigation of the transcriptome specific for the mouse synaptosome and aging-related alterations in lncRNAs profiles; in collaboration with Prof. Thomas Arendt and Dr. Uwe Ueberham from Paul Flechsig Institute for Brain Research, University of Leipzig, Germany.

LABORATORY MEMBERS

• Si-Mei Xu, PhD candidate

• Konii Takenaka, PhD candidate

• Yuning Cheng, PhD candidate

• Yulan Gao, PhD candidate

• Grace Lindner, PhD candidate

• Kristina Santucci, Honours student

• Zayyaf Aazan Hasnie, Honours student

• Divyanshi Mathur, Honours student

• Sania Parekh, Talented Students Program

• Rose Mitsui, Talented Students Program

• Bronte Critchley, Talented Students Program

SELECTED PUBLICATIONS

1. Cheng Y, Xu SM, Takenaka K, Lindner G, Curry-Hyde A, Janitz M. A unique circular RNA expression pattern in the peripheral blood of myalgic encephalomyelitis/chronic fatigue syndrome patients. Gene. 2023 Aug 15;877:147568.

2. Takenaka K, Olzomer EM, Hoehn KL, Curry-Hyde A, Jun Chen B, Farrell R, Byrne FL, Janitz M. Investigation of circular RNA transcriptome in obesity-related endometrial cancer. Gene. 2023 Mar 1;855:147125.
3. Aquilina-Reid C, Brennan S, Curry-Hyde A, Teunisse GM, The Nygc Als Consortium, Janitz M. Circular RNA Expression and Interaction Patterns Are Perturbed in Amyotrophic Lateral Sclerosis. Int J Mol Sci. 2022 Nov 24;23(23):14665.
4. Takenaka K, Curry-Hyde A, Olzomer EM, Farrell R, Byrne FL, Janitz M. Investigation of Transcriptome Patterns in Endometrial Cancers from Obese and Lean Women. Int J Mol Sci. 2022 Sep 29;23(19):11471.
5. Leitner DF, Devore S, Laze J, Friedman D, Mills JD, Liu Y, Janitz M, Anink JJ, Baayen JC, Idema S, van Vliet EA, Diehl B, Scott C, Thijs R, Nei M, Askenazi M, Sivathamboo S, O'Brien T, Wisniewski T, Thom M, Aronica E, Boldrini M, Devinsky O. Serotonin receptor expression in hippocampus and temporal cortex of temporal lobe epilepsy patients by postictal generalized electroencephalographic suppression duration. Epilepsia. 2022 Nov;63(11):2925-2936.
6. Xu SM, Curry-Hyde A, Sytnyk V, Janitz M. RNA polyadenylation patterns in the human transcriptome. Gene. 2022 Mar 30;816:146133.
7. Leitner DF, Mills JD, Pires G, Faustin A, Drummond E, Kanshin E, Nayak S, Askenazi M, Verducci C, Chen BJ, Janitz M, Anink JJ, Baayen JC, Idema S, van Vliet EA, Devore S, Friedman D, Diehl B, Scott C, Thijs R, Wisniewski T, Ueberheide B, Thom M, Aronica E, Devinsky O. Proteomics and Transcriptomics of the Hippocampus and Cortex in SUDEP and High-Risk SUDEP Patients. Neurology. 2021 May 25;96(21):e2639-e2652.
8. Kozlova I, Sah S, Keable R, Leshchyns'ka I, Janitz M, Sytnyk V. Cell Adhesion Molecules and Protein Synthesis Regulation in Neurons. Front Mol Neurosci. 2020 Nov 12;13:592126.
9. Gray LG, Mills JD, Curry-Hyde A, Devore S, Friedman D, Thom M, Scott C, Thijs RD, Aronica E, Devinsky O, Janitz M. Identification of Specific Circular RNA Expression Patterns and MicroRNA Interaction Networks in Mesial Temporal Lobe Epilepsy. Front Genet. 2020 Sep 25;11:564301.
10. Curry-Hyde A, Gray LG, Chen BJ, Ueberham U, Arendt T, Janitz M. Cell type- specific circular RNA expression in human glial cells. Genomics. 2020 Nov;112(6):5265-5274.
11. Curry-Hyde A, Ueberham U, Chen BJ, Zipfel I, Mills JD, Bochmann J, Jendrek R, Takenaka K, Kirazov L, Kirazov E, Jünger J, Brückner MK, Arendt T, Janitz M. Analysis of the Circular Transcriptome in the Synaptosomes of Aged Mice. Neuroscience. 2020 Nov 21;449:202-213.
12. Mills JD, van Vliet EA, Chen BJ, Janitz M, Anink JJ, Baayen JC, Idema S, Devore S, Friedman D, Diehl B, Thom M, Scott C, Thijs R, Aronica E, Devinsky O. Coding and non-coding transcriptome of mesial temporal lobe epilepsy: Critical role of small non-coding RNAs. Neurobiol Dis. 2020 Feb;134:104612.
13. Chen, Byrne FL, Takenaka K, Modesitt SC, Olzomer EM, Mills JD, Farrell R, Hoehn KL, Janitz, M. (2017) Analysis of the circular RNA transcriptome in endometrial cancer. Oncotarget 9:5786–5796.
14. Chen BJ, Byrne FL, Takenaka K, Modesitt SC, Olzomer EM, Mills JD, Farrell R, Hoehn KL, Janitz M (2017) Transcriptome landscape of long intergenic non-coding RNAs in endometrial cancer. Gynecol Oncol 47:654-662.
15. Chen BJ, Mills JD, Takenaka K, Bliim N, Halliday GM, Janitz M (2016) Characterization of circular RNAs landscape in multiple system atrophy brain. J Neurochem 139:485-496.
16. Mills JD, Kavanagh T, Kim WS, Chen BJ, Waters PD, Halliday GM, Janitz M (2015) High expression of long intervening non-coding RNA OLMALINC in the human cortical white matter is associated with regulation of oligodendrocyte maturation. Mol Brain 8:2.
17. Mills JD, Kim WS, Halliday GM, Janitz M (2015) Transcriptome analysis of grey and white matter cortical tissue in multiple system atrophy. Neurogenetics 16:107-122.
18. Twine NA, Janitz K, Wilkins MR, Janitz M (2011) Whole transcriptome sequencing reveals gene expression and splicing differences in brain regions affected by Alzheimer's disease. PLoS One 6:e16266.
19. Warnatz HJ, Querfurth R, Guerasimova A, Cheng X, Haas SA, Hufton AL, Manke T, Vanhecke D, Nietfeld W, Vingron M, Janitz M, Lehrach H, Yaspo ML (2010) Functional analysis and identification of cis-regulatory elements of human chromosome 21 gene promoters. Nucleic Acids Res 38:6112-6123.
20. Niesner U, Albrecht I, Janke M, Doebis C, Loddenkemper C, Lexberg MH, Eulenburg K, Kreher S, Koeck J, Baumgrass R, Bonhagen K, Kamradt T, Enghard P, Humrich JY, Rutz S, Schulze-Topphoff U, Aktas O, Bartfeld S, Radbruch H, Hegazy AN, Löhning M, Baumgart DC, Duchmann R, Rudwaleit M, Haeupl T , Gitelman I, Krenn V, Gruen J, Sieper J, Zeitz M, Wiedenmann B, Zipp F, Hamann A, Janitz M, Scheffold A, Burmester GR, Chang HD, Radbruch A (2008) Autoregulation of Th1-mediated inflammation by twist1. J Exp Med 205:1889-1901.
21. Cowell LG, Kepler TB, Janitz M, Lauster R, Mitchison NA (1998) The distribution of variation in regulatory gene segments, as present in MHC class II promoters. Genome Res 8:124-134.

PROFESSIONAL EXPERIENCE

• 2009 - current, Senior Lecturer, School of BABS, լ�и��� Sydney
• 2000 - 2008 Group Leader, Max Planck Institute for Molecular Genetics (Department Prof. Hans Lehrach), Berlin, Germany
• 1998 -1999 Postdoctoral Fellow, German Centre for Rheumatism Research, Berlin, Germany

HONOURS & AWARDS

• Member of Editorial Board, Genomics (since 2020)
• Member of Editorial Board, Non-coding RNA (since 2020)
• Guest Editor, Special Issue Neuroscience Letters (2020)
• Guest Scientist at Paul Flechsig Institute for Brain Research, University of Leipzig (since 2017)
• NSW Brain Banks Scientific Review Committee member (since 2017)
• Member of Editorial Board, Gene (since 2014)
• Member of Editorial Board - Frontiers in Epigenomics (2011 - 17)
• Consultant to German Federal Institute for Risk Assessment regarding application of genomic methods as an alternative to animal experiments (2009)
• Consultant to Gerson Lehrman Group concerning next-generation sequencing technologies and future development perspectives (2007 – 09)
• Consultant to the German Federal Ministry of Education and Research concerning development of funding schemes in the field of genomic technologies (2007)